A broad-range PCR technique for the diagnosis of infective endocarditis
نویسندگان
چکیده
منابع مشابه
A broad-range PCR technique for the diagnosis of infective endocarditis.
Infective endocarditis (IE) remains a severe and potentially fatal disease demanding sophisticated diagnostic strategies for detection of the causative microorganisms. The aim of the present study was to develop a broad-range 16S ribosomal RNA gene polymerase chain reaction in the routine diagnostic of IE for the early diagnosis of fatal disease. A broad-range PCR technique was selected and eva...
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Infective endocarditis can complicate with ischemic stroke. Neurological complications develop in 20-40% of all patients with infective endocarditis. These are mainly due to vegetation embolism.This report represents a case of 30 years old female patient who presented with features of heart failure New York Heart Association (NYHA) class IV with infective endocarditis and left hemiparesis. On e...
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Broad-range PCR amplification of part of the 16S rRNA gene followed by single-strand sequencing was applied to samples of 18 resected heart valves from patients with infective endocarditis. The PCR results were compared with those of cultures of valves and with those of previous blood cultures. For two patients there was agreement with the cultures of the valves; for nine patients there was agr...
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A 76-year-old man was admitted to a community hospital due to a persistent high fever. He became afebrile after the administration of broad-spectrum antibiotics, but developed heart failure due to progressive aortic and mitral valve insufficiency and was transferred to our hospital. Although sequential blood cultures were negative, a broad-range polymerase chain reaction targeting the bacterial...
متن کاملUtility of a Direct 16S rDNA PCR and Sequencing for Etiological Diagnosis of Infective Endocarditis
BACKGROUND Cases of infective endocarditis (IE) require prompt etiological diagnosis for effective treatment. Molecular methods can aid in rapid and reliable diagnosis of culture-negative IE cases. We evaluated the utility of 16S rDNA PCR and sequencing in determining the causative agents of IE in valve tissues, especially when specimens were obtained after initiation of antimicrobial therapy. ...
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ژورنال
عنوان ژورنال: Brazilian Journal of Microbiology
سال: 2018
ISSN: 1517-8382
DOI: 10.1016/j.bjm.2017.03.019